The aims of this study are to examine the concentration and identification of airborne organism at exhibit hall and storage in museums and to provide fundamental data to protect cultural assests from damage. This study surveyed the concentration of airborne bacteria and fungi at exhibit hall and storage in museums located 4 metropolitans from July to October, 2007 and literature data on airborne microbes of museums in Korea that were publiced in relevant Korean journals. The concentrations of airborne bacteria and fungi were higher than those in storage. Pseudomonas spp. and Bacillus spp for bacteria and Aspergillus spp. and Penicillium spp. for fungi were shown mainly in museums. Pooled average concentration of airborne bacteria in museums was 34.2±10.1CFU/㎥ for exhibit hall and 17.2±10.0CFU/㎥ for storage, respectively. Pooled average concentration of airborne fungi in museum was 26.0±11.2CFU/㎥ for exhibit hall and 16.6±11.0CFU/㎥ for storage, respectively. Keywords：Museum, Exhibit hall, Storage, Bacteria, Fungi