When radiation is irradiated onto biological tissue, the occurrence of biological change has been already demonstrated and the change of blood composition and chromosome abnormality could be suggested as the representative biological markers which can identify the disturbance caused by radiation exposure. Recently, radiation treatment is performed for the treatment of cancer. Since the protection of blood within normal tissue is very important, the development of radioprotective has been made continuously to protect normal tissues. Squalene is a very important precursor in the biosynthesis of cholesterol. It is the hydrocarbon contained in the liver of deep sea sharks and in olive oils, and large quantity of it is contained in skin, abdominal fat tissue, subcutaneous fat tissue, lymphatic gland, pancreas and in heart muscle. Because of 6 double bonds in SQ, it could easily bind with oxygen ions to have the hazardous oxygen removal capacity along with the effects of scar treatment, blood vessel expansion, inhibitory action of arteriosclerosis, and has been reported to have the effects on myocardial infarction and liver disease, treatment for burn. It has been reported to have active effect of fibroblast. Therefore, the current study was conducted to know the change of total protein, albumin, total cholesterol and glucose in serum after the injection of whole body radiation of SQ in mouse. Keywords：Irradiation, biological change, Radioprotective, Squalene