Bioaerosols, airborne particles of biological origin, are omnipresent in the common surroundings of humans. In antimicrobial filter system, as particles or organic materials are deposited on the filter surface, the bacteria come into contact with the antimicrobial material less, and thus they can easily grow on particles or organic materials. We selected a carbon fiber ionizer which generated air ions as antimicrobial agent. Antimicrobial effect of carbon fiber ionizer on the outlet air and condensate water was tested using application of carbon fiber ionizer in a small-scale commercial air conditioner. In conclusion, the application of carbon fiber ionizer in the air conditioner showed more increasing antimicrobial efficiency. Keywords：Air conditioner, Carbon fiber ionizer, Bioaerosol, Antimicrobial